Nicole de Boer began her acting career as a child in her native Toronto. A shy child, who one day came home, and, to the astonishment of her family, told them she had gotten the lead in the play, "The Wizard of Oz". With the blessing of her parents, her grandmother helped her to find an agent and shortly afterward, she made her television debut in a Christmas Special starring Red Skelton and Vincent Price. Throughout her childhood, Nicole was active in commercials and theatre.

At age seventeen, she was cast as a series regular in the CBC drama 9B. Nicole's numerous television credits include: Beyond Reality, First Resort, Catwalk, The Kids in the Hall, The Outer Limits, PSI Factor: Chronicles of the Paranormal, Maniac Mansion and Deepwater Black (aka Mission Genesis).

On film, she was featured in Kids in the Hall: Brain Candy, co-starred in the film National Lampoon's Senior Trip with Matt Frewer and Tommy Chong and was in the critically received Sci-Fi movie, Cube. While filming the series Dooley Gardens in Newfoundland, she got the call and was cast in the 7th and final season of Star Trek: Deep Space Nine, replacing Terry Farrell as the Dax symbiont host.
Her last name in Dutch translates into 'the Farmer'. Appeared with Torri Higginson in four different productions: Family Pictures, TekWar and Family of Cops III: Under Suspicion. Has starred in two different science fiction series co-created by Michael Piller in which her character was part of a love triangle: Star Trek: Deep Space Nine and Stephen King's Dead Zone.